Twin Cam Harley fuel filter removal is one of the most important service items to keep on top of. Your year and model manual will tell you when it is recommended.
If it recommends performing a fuel tank filter replacement at 25,000 miles, it does not take into account where you have been riding and the fuels you have been using. If you are experiencing some fuel delivery related issues, this could be one of the causes. You will need new gaskets, filter, and cover bolts.
Take your time and do not force anything or yank anything in the Harley fuel tank. You do not want to bend or break anything. Cover painted pieces to avoid unwanted paint damage.
This video is also valuable if you have to replace your fuel supply line.
Now put it back together with this video:
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